Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons

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dc.contributor.author Quiroga, Maria Florencia
dc.contributor.author Angerami, Matias Tomas
dc.contributor.author Santucci, Natalia
dc.contributor.author Ameri, Diego
dc.contributor.author Francos, Jose Luis
dc.contributor.author Wallach, Jorge
dc.contributor.author Sued, Omar
dc.contributor.author Cahn, Pedro
dc.contributor.author Salomón, Horacio
dc.contributor.author Bottasso, Oscar
dc.date.accessioned 2014-03-26T19:08:22Z
dc.date.available 2014-03-26T19:08:22Z
dc.date.issued 2012-03-04
dc.identifier.citation Quiroga MF, Angerami MT, Santucci N, Ameri D, Francos JL, et al. (2012) Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons. PLoS ONE 7(3): e33061. doi:10.1371/journal.pone.0033061 es
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/2133/3080
dc.description.abstract Tuberculosis (TB) remains the most frequent cause of illness and death from an infectious agent, and its interaction with HIV has devastating effects. We determined plasma levels of dehydroepiandrosterone (DHEA), its circulating form DHEA-suphate (DHEA-s) and cortisol in different stages of M. tuberculosis infection, and explored their role on the Th1 and Treg populations during different scenarios of HIV-TB coinfection, including the immune reconstitution inflammatory syndrome (IRIS), a condition related to antiretroviral treatment. DHEA levels were diminished in HIV-TB and HIV-TB IRIS patients compared to healthy donors (HD), HIV+ individuals and HIV+ individuals with latent TB (HIV-LTB), whereas dehydroepiandrosterone sulfate (DHEA-s) levels were markedly diminished in HIV-TB IRIS individuals. HIV-TB and IRIS patients presented a cortisol/DHEA ratio significantly higher than HIV+, HIV-LTB and HD individuals. A positive correlation was observed between DHEA-s and CD4 count among HIV-TB individuals. Conversely, cortisol plasma level inversely correlated with CD4 count within HIV-TB individuals. M. tuberculosis-specific Th1 lymphocyte count was increased after culturing PBMC from HIV-TB individuals in presence of DHEA. We observed an inverse correlation between DHEA-s plasma level and Treg frequency in co-infected individuals, and CD4+FoxP3+ Treg frequency was increased in HIV-TB and IRIS patients compared to other groups. Strikingly, we observed a prominent CD4+CD25-FoxP3+ population across HIV-TB and HIV-TB IRIS patients, which frequency correlated with DHEA plasma level. Finally, DHEA treatment negatively regulated FoxP3 expression without altering Treg frequency in co-infected patients. These data suggest an enhancing role for DHEA in the immune response against M. tuberculosis during HIV-TB coinfection and IRIS. es
dc.format application/pdf
dc.language.iso en es
dc.publisher PLOS (Public Library of Science) es
dc.rights openAccess
dc.subject Tuberculosis es
dc.subject Blood plasma es
dc.subject HIV es
dc.subject Hydrocortisone es
dc.subject Immune response es
dc.subject Mycobacterium tuberculosis es
dc.subject Regulatory T cells es
dc.subject T cells es
dc.title Dynamics of Adrenal Steroids Are Related to Variations in Th1 and Treg Populations during Mycobacterium tuberculosis Infection in HIV Positive Persons es
dc.type artículo
dc.type article
dc.type publishedVersion
dc.description.peerreviewed Peer reviewed es
dc.relation.publisherversion http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033061 es
dc.description.affiliation Fil: Quiroga, Maria Florencia. Universidad de Buenos Aires. Facultad de Medicina. Centro Nacional de Referencia para el Sida (CNRS); Argentina.
dc.description.affiliation Fil: Angerami, Matias Tomas. Universidad de Buenos Aires. Facultad de Medicina. Centro Nacional de Referencia para el Sida (CNRS); Argentina.
dc.description.affiliation Fil: Santucci, Natalia. Universidad Nacional de Rosario, Facultad de Ciencias Médicas, Instituto de Inmunología; Argentina.
dc.description.affiliation Fil: Bottasso, Oscar. Universidad Nacional de Rosario, Facultad de Ciencias Médicas, Instituto de Inmunología; Argentina.


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