Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus

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dc.creator Pellieri, Carlos Martín
dc.creator Taddia, Antonela
dc.creator Loureiro, Dana Belén
dc.creator Bortolato, Santiago Andrés
dc.creator Tubio, Gisela
dc.date.accessioned 2022-04-08T14:45:23Z
dc.date.available 2022-04-08T14:45:23Z
dc.date.issued 2022-01-24
dc.identifier.issn 2352-1864 es
dc.identifier.uri http://hdl.handle.net/2133/23373
dc.description Hemicellulose is a major component of plant cell walls and xylan is the most predominant polysaccharide. Xylan degrading enzymes integrate the xylanolytic system. Xylanolytic enzymes were produced by fermentation by Aspergillus niger and Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type of fermentation used (SmF or SSF), type of leaves of S. argentinensis (green or senescent) and conidia final concentration for xylanase production were screened and optimized. The main results showed that the highest levels of xylanolytic enzyme production were obtained by 1 × 105 conidia/mL of A. niger in SmF at 96 h, 30ºC, with a mixture of 20.20% of senescent and 79.80% of green leaves. The xylanase specific activity obtained was 62 U/mg, higher than the activity obtained (23 U/mg) in previous work (Taddia et al., 2019), and the concentration of xylanolytic production over that of glucanase activity was maximized five times. The optimized enzymatic extract obtained was characterized by LC-MS and HPLC of carbohydrates. Six enzymes were identified as constituents of the xylanolytic complex and seven carbohydrates. Moreover, the xylanolytic enzyme extract was stable for 30 days at 20 °C. Thus, S. argentinensis can be used within the framework of a circular economy, rendering a synergistic combination of the xylanolytic enzymes with industrial applications. es
dc.description.sponsorship Ministerio de Ciencia, Tecnología e Innovación Productiva de Santa Fe: IO2018009
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PUE BD20160041CO
dc.description.sponsorship Agencia Nacional de Promoción Científica y Tecnológica: PICT-II-B-2018 2054
dc.description.sponsorship Secretaría de Ciencia y Tecnología de la Universidad Nacional de Rosario: 80020180100033UR
dc.description.sponsorship Secretaría de Ciencia y Tecnología de la Universidad Nacional de Rosario: 80020180100036UR
dc.format application/pdf
dc.format.extent 1-14 es
dc.language.iso eng es
dc.publisher Elsevier es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Bioprocesses es
dc.subject Lignocellulosic Biomass es
dc.subject Fungal Fermentation es
dc.subject Enzyme Statistical Design Production es
dc.subject Sustainability es
dc.subject Circular Economy es
dc.title Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder Pellieri, Carlos Martín es
dc.rights.holder Taddia, Antonela es
dc.rights.holder Loureiro, Dana Belén es
dc.rights.holder Bortolato, Santiago Andrés es
dc.rights.holder Tubio, Gisela es
dc.relation.publisherversion https://www.sciencedirect.com/science/article/pii/S2352186422000165?via%3Dihub es
dc.rights.text Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) es
dc.citation.title Environmental Technology & Innovation es
dc.citation.volume 26 es
dc.description.fil Fil: Pellieri, Carlos Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina. es
dc.description.fil Fil: Taddia, Antonela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina. es
dc.description.fil Fil: Loureiro, Dana Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina. es
dc.description.fil Fil: Bortolato, Santiago Andrés . Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química de Rosario (IQUIR-CONICET). Argentina. es
dc.description.fil Fil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina. es
dc.type.collection articulo
dc.type.version publishedVersion es


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