Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain

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dc.creator Cerminati, Sebastián
dc.creator Leroux, Mélanie
dc.creator Anselmi, Pablo Ariel
dc.creator Peirú, Salvador
dc.creator Alonso, Juan C.
dc.creator Priem, Bernard
dc.creator Menzella, Hugo G.
dc.date.accessioned 2022-03-29T16:13:31Z
dc.date.available 2022-03-29T16:13:31Z
dc.date.issued 2021-04-05
dc.identifier.issn 1432-0614 es
dc.identifier.uri http://hdl.handle.net/2133/23283
dc.description Hyaluronic acid (HA) is a high value glycosaminoglycan mostly used in health and cosmetic applications. Commercial HA is produced from animal tissues or in toxigenic bacteria of the genus Streptococcus grown in complex media, which are expensive and raise environmental concerns due to the disposal of large amounts of broth with high organic loads. Other microorganisms were proposed as hosts for the heterologous production of HA, but the methods are still costly. The extraordinary capacity of this biopolymer to bind and retain water attracts interest for large-scale applications where biodegradable materials are needed, but its high cost and safety concerns are barriers for its adoption. Bacillus subtilis 3NA strain is prototrophic, amenable for genetic manipulation, GRAS, and can rapidly reach high cell densities in salt-based media. These phenotypic traits were exploited to create a platform for biomolecule production using HA as a proof of concept. First, the 3NA strain was engineered to produce HA; second, a chemically defined medium was formulated using commodity-priced inorganic salts combined at the stoichiometric ratios needed to build the necessary quantities of biomass and HA; and third, a scalable fermentation process, where HA can be produced at the maximum volumetric productivity (VP), was designed. A comparative economic analysis against other methods indicates that the new process may increase the operating profit of a manufacturing plant by more than 100%. The host, the culture medium, and the rationale employed to develop the fermentation process described here, introduce an IP free platform that could be adaptable for production of other biomolecules. es
dc.format application/pdf
dc.format.extent 3075–3086 es
dc.language.iso eng es
dc.publisher Springer es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.subject Hyaluronic acid es
dc.subject Green chemistry es
dc.subject Bacillus subtilis es
dc.subject Fermentation es
dc.title Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder Cerminati, Sebastián es
dc.rights.holder Leroux, Mélanie es
dc.rights.holder Anselmi, Pablo Ariel es
dc.rights.holder Peirú, Salvador es
dc.rights.holder Alonso, Juan C. es
dc.rights.holder Priem, Bernard es
dc.rights.holder Menzella, Hugo G. es
dc.relation.publisherversion https://doi.org/10.1007/s00253-021-11246-6
dc.relation.publisherversion https://link.springer.com/article/10.1007/s00253-021-11246-6#Ack1
dc.rights.text Attribution 4.0 International (CC BY 4.0) es
dc.citation.title Applied Microbiology and Biotechnology es
dc.description.fil Fil: Cerminati, Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina. es
dc.description.fil Fil: Leroux, Mélanie. Université Grenoble Alpes. Centre National de la Recherche Scientifique. Centre de Recherches sur les Macromolécules Végétales; France. es
dc.description.fil Fil: Anselmi, Pablo Ariel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina. es
dc.description.fil Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina. es
dc.description.fil Fil: Alonso, Juan C. Centro Nacional de Biotecnología. Department of Microbial Biotechnology; Spain. es
dc.description.fil Fil: Priem, Bernard. Université Grenoble Alpes. Centre National de la Recherche Scientifique. Centre de Recherches sur les Macromolécules Végétales; France. es
dc.description.fil Fil: Menzella, Hugo G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina. es
dc.type.collection articulo
dc.type.version publishedVersion es


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