Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

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dc.creator Ordoñez, Omar F.
dc.creator Lanzarotti, Esteban
dc.creator Kurth, Daniel
dc.creator Cortez, Néstor
dc.creator Farías, María E.
dc.creator Turjanski, Adrian G.
dc.date.accessioned 2020-12-17T13:37:11Z
dc.date.available 2020-12-17T13:37:11Z
dc.date.issued 2015-07-22
dc.identifier.issn 2296-665X es
dc.identifier.uri http://hdl.handle.net/2133/19499
dc.description Arsenic exists in natural systems in a variety of chemical forms, including inorganic arsenite (As [III]) and arsenate (As [V]). The majority of living organisms have evolved various mechanisms to avoid occurrence of arsenic inside the cell due to its toxicity. Common core genes include a transcriptional repressor ArsR, an arsenate reductase ArsC, and arsenite efflux pumps ArsB and Acr3. To understand arsenic resistance we have performed arsenic tolerance studies, genomic and bioinformatic analysis of two Exiguobacterium strains, S17 and N139, from the high-altitude Andean Lakes. In these environments high concentrations of arsenic were described in the water due to a natural geochemical phenomenon, therefore, these strains represent an attractive model system for the study of environmental stress and can be readily cultivated. Our experiments show that S17 has a greater tolerance to arsenite (10 mM) than N139, but similar growth in arsenate (150 mM). We sequenced the genome of the two Exiguobacterium and identified an acr3 gene in S17 as the only difference between both species regarding known arsenic resistance genes. To further understand the Acr3 we modeled the 3D structure and identified the location of relevant residues of this protein. Our model is in agreement with previous experiments and allowed us to identify a region where a relevant cysteine lies. This Acr3 membrane efflux pump, present only in S17, may explain its increased tolerance to As(III) and is the first Acr3-family protein described in Exiguobacterium genus. es
dc.description.sponsorship Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 0365/12 es
dc.description.sponsorship Fondo para la Investigación Científica y Tecnológica (FONCYT): PICT 1788 y 2805 es
dc.format application/pdf
dc.format.extent 50 es
dc.language.iso eng es
dc.publisher Frontiers es
dc.rights openAccess es
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ *
dc.subject Arsenic resistance es
dc.subject Exiguobacterium, es
dc.subject Membrane proteins es
dc.subject Sequencing es
dc.subject ACR3 es
dc.title Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump es
dc.type article
dc.type artículo
dc.type publishedVersion
dc.rights.holder Ordoñez, Omar F. es
dc.rights.holder Lanzarotti, Esteban es
dc.rights.holder Kurth, Daniel es
dc.rights.holder Cortez, Néstor es
dc.rights.holder Farías, María E. es
dc.rights.holder Turjanski, Adrian G. es
dc.rights.holder Universidad Nacional de Rosario es
dc.relation.publisherversion https://www.frontiersin.org/articles/10.3389/fenvs.2015.00050/full#h8 es
dc.relation.publisherversion https://doi.org/10.3389/fenvs.2015.00050 es
dc.rights.text Attribution 4.0 International (CC BY 4.0) es
dc.citation.title Frontiers in Environmental Science es
dc.citation.volume 3 es
dc.description.fil Fil: Ordoñez, Omar F. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina. es
dc.description.fil Fil: Lanzarotti, Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE-CONICET); Argentina. es
dc.description.fil Fil: Kurth, Daniel. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina. es
dc.description.fil Fil: Cortez, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. es
dc.description.fil Fil: Farías, María E. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina. es
dc.description.fil Fil: Turjanski, Adrian G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE-CONICET); Argentina. es
dc.type.collection articulo
dc.type.version publishedVersion es


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